Binding versus triggering riboswitches.
نویسنده
چکیده
In this issue of Chemistry & Biology, Trausch and Batey report a discrepancy between ligand binding affinity and the effect of transcription termination in a THF riboswitch, raising some important questions about our current understanding of ligand-dependent RNA switches.
منابع مشابه
A flow cytometry-based screen for synthetic riboswitches
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PURPOSE Riboswitches, as noncoding RNA sequences, control gene expression through direct ligand binding. Sporadic reports on the structural relation of riboswitches with ribosomal RNAs (rRNA), raises an interest in possible similarity between riboswitches and rRNAs evolutionary origins. Since aminoglycoside antibiotics affect microbial cells through binding to functional sites of the bacterial ...
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Riboswitches are intricate, metabolite-binding RNA structures found in the non-coding regions of mRNA. Allosteric changes in the riboswitch that are induced by metabolite binding are harnessed to control the genes of a variety of essential metabolic pathways in eubacteria and in some eukaryotes. In this chapter, we describe an RNA structure analysis technique called "in-line probing." This assa...
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عنوان ژورنال:
- Chemistry & biology
دوره 21 2 شماره
صفحات -
تاریخ انتشار 2014